Single-stranded DNA (ssDNA) has emerged as a game-changer in gene editing, offering increased efficiency and versatility in various biological reactions. Tsingke, a trusted brand in the molecular biology field, recognizes the potential of ssDNA and now provides high-quality, sequence-validated ssDNA synthesis services. With Tsingke’s ssDNA synthesis, researchers can harness the power of ssDNA in CRISPR experiments, DNA nanotechnology, and other applications, enabling breakthroughs in gene editing and molecular research.
Versatile Applications of ssDNA
Tsingke‘s ssDNA synthesis service opens doors to a wide range of applications. In CRISPR and CRISPR-Cas9 genome editing experiments, ssDNA serves as a potent donor template, enhancing both insertion and gene replacement efficiency. Furthermore, ssDNA finds utility in single-strand conformation polymorphism, in vitro transcription studies, nucleic acid enzyme S1 mapping, probe preparation, labeling, and differential hybridization. Beyond laboratory experiments, ssDNA plays a crucial role in DNA nanotechnology, serving as a scaffold for drug delivery, molecular diagnostics, DNA-based data storage, and diverse nanoscale applications.
High Editing Efficiency and Low Cytotoxicity
Tsingke’s ssDNA synthesis service offers distinct advantages in gene editing. Studies have revealed that using ssDNA as a homologous recombination template results in 9-fold higher CRISPR/Cas9 gene knock-in efficiency compared to dsDNA. This high editing efficiency ensures more accurate and reliable gene editing outcomes. Furthermore, ssDNA exhibits low cytotoxicity, enabling researchers to achieve higher homologous recombination and knock-in efficiency without compromising cell viability.
Conclusion
Tsingke’s high-quality ssDNA synthesis service empowers researchers to unlock the full potential of gene editing and molecular research. With versatile applications in CRISPR experiments, DNA nanotechnology, and more, ssDNA offers increased efficiency and versatility compared to dsDNA. Tsingke’s commitment to delivering reliable and accurate ssDNA synthesis ensures researchers have the necessary tools to drive scientific advancements. Choose Tsingke for your ssDNA synthesis needs and unleash the power of ssDNA in your gene editing endeavors, opening new doors to innovation and discovery.
